3D High-Content Screening of Organoids for Drug Discovery

Linfeng Li and D.V. LaBarbera – Anschutz Medical Campus, University of Colorado

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Abstract: We are entering a new era of biomedical research that is driven by the demand for more effective therapeutics to prevent and treat human disease. Organoids, cultured ex vivo, are the future of this new era of biomedical research and are poised to replace preclinical 2D cell models, and in some cases animal models of human disease. Therefore, the drug discovery and development pipeline is retooling high-throughput technologies to accommodate organoids as the model of choice. In particular, the marriage of high-content screening (HCS) with organoid models for drug discovery will be a critical component in this new era of drug development. This book chapter is focused on the state-of-the-art HCS technology and how this technology is being retooled for drug discovery and development with human organoids.

Keywords: 3D imaging technology, 3D tissue culture, Drug discovery, Extracellular matrix, High-content screening, High-throughput imaging, High-throughput screening, Image analysis software, Microenvironment, Multicellular tumor spheroids, Organoids, Organotypic, Pluripotent stem cells, Precision medicine, Spheroids

Cell Type(s): All Cell Lines