Plasma treatment is one of the more effective methods for adequate surface modification. If you do not have access to a plasma machine in your lab, you can also use PDL coating. Please refer to our manual or contact our support team for details of each protocol.
Read MoreWe recommend similar protocol for SMCs as we do for cardiomyocytes. For coating, however, we normally used Matrigel, gelatin or Collagen I.
Read MoreThis depends on cell source and the stage the cells are in. However, we recommend the same protocols for stem cell-derived somatic cells.
Read MoreAs with any cultureware, optimal seeding density on ANFS can vary according to cell type and the purpose of the experiment. However, we recommend researchers use exactly the same cell density that they use for normal 35mm dishes, 6-well dishes and so on. Protocol information is provided on the ANFS product pages.
Read MoreNo, ANFS is already gamma sterilized and plasma treated to promoted cellular adhesion. However, researchers may choose to deposit additional surface treatments depending on their exact cell type. Fibronectin, collagen, and PLL are all common surface treatment choices.
Read MoreIdeally, no if you follow the protocol provided. However, there is a possibility that cells can attach on to plastic plate bottom if you don’t follow the protocol.
Read MoreYes. The working volume is 3 mL. Also, amount and duration for cell media change should be the same as with normal 6-well or single-well culture.
Read MoreYes, we recommend that our customers seed cells using the same density that they do on conventional 6-well plates.
Read MoreWe have suggestions for cell seeding density for several of the commercially available cell lines. These information will be provided to you by our application scientific support team upon your request. In general, for commercially available cell lines, the cell seeding density used on conventional cultureware works with NanoSurface Cultureware as well. For any suggestions with in-house cell lines, please contact our support team.
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